In his PhD thesis, Christian demonstrates how hyperpolarization can be used the increase the sensitivity of single scan solution 13C-NMR with up to four orders of magnetitude.
PhD student Christian Kjeldsen succesfully defended his PhD thesis on Structural elucidation of polysaccharides and investigations of enzymatic synthesis of oligosaccharides using NMR spectroscopy on 16 February 2018.
Christian has worked in part as a member of Center for Hyperpolarization in Magnetic Resonance (HYPERMAG), contributing by gaining insight into the inner workings of enzymes in real time using hyperpolarized Nuclear Magnetic Resonance (NMR) and thus reaching a better understanding of the chemistry and biochemistry of enzymatic carbohydrate conversion.
His thesis, among other insights, shows how hyperpolarization by dissolution dynamic nuclear polarisation (dDNP) can be used to increase the sensitivity of single scan solution 13C-NMR with up to four orders of magnitude. Using dDNP-NMR, previously undescribed short-lived transglycosylation products of the enzyme were observed, and the hydrolysis and transglycosylation rates were determined using a kinetic model.
Examiners were
- Associate Professor Pernille Rose Jensen, DTU Electrical Engineering
- Associate Professor Flemming Hofmann Larsen, University of Copenhagen
- Senior Lecturer Jesus Angulo, University of East Anglia, United Kingdom
Christian's PhD project is funded by Novo Nordisk Foundation. His supervisors are Professor Jens Øllgaard Duus and Senior Researcher Sebastian Meier from DTU Chemistry.
You can find Christian's publications at DTU's research repository DTU Orbit, where his PhD thesis will shortly be made available as well.
(Caption: From left to right, Christian Kjeldsen, Jesus Angulo, Pernille Rose Jensen, Flemming Hoffmann Larsen, and Sebastian Meier).